Czech and Slovak Ophthalmology 328 10.31348/2024/24 Original article Choroidal Thickness and Central Macular Thickness Measurements with Cirrus HD-OCT in Healthy Individuals in the Turkish Population Akyüz Tuba Kara https://orcid.org/0009-0002-1950-7410 Gürağaç Fatma Betül https://orcid.org/0009-0004-8860-1472 Totan Yüksel https://orcid.org/0009-0001-7003-6995 19 6 2024 1 5

Aim: This research was conducted to determine the normal values of choroidal thickness in healthy individuals and to evaluate the relationship between this thickness and age, gender, refraction, axial length and average macular thickness using OCT. Material and Method: In the study, the right eyes of 400 healthy individuals (234 women, 166 men) between the ages of 4 and 70 years, who applied to the Department of Ophthalmology outpatient clinic for examination, were evaluated. Results: Macular thickness, macular volume, and foveal thickness were found to be 249.12 ±21.32 µm, 9.98 ±0.5 µm3 and 280 ±13.45 µm, respectively. According to linear regression analysis, a negative correlation was detected between age and subfoveal choroidal thickness (p < 0.05). It was determined that foveal thickness, retinal volume and average retinal thickness were higher in men, and foveal thickness increased with age (p < 0.05). Conclusion: As a result of the research, it was determined that age is an important factor affecting choroidal thickness. It is thought that, in future, improving in vivo imaging of the choroid and measuring choroidal thickness using OCT will facilitate understanding of the pathophysiological basis of many ophthalmological diseases.

 Cirrus HD-OCT choroidal thickness central macular thickness age References Wangsa-Wirawan ND, Linsenmeier RA. Retinal oxygen: fundamental and clinical aspects. Arch Ophthalmol.2003;121: 547-557. Gemenetzi M, De Salvo G, Lotery AJ. Central serous chorioretinopathy: an update on pathogenesis and treatment. Eye [Lond].2010;24:1743-1756. Stepanov A. Current vıew of the spectrum of pachychoroıd dıseases. a revıew. Cesk Slov Oftalmol. 2023;4:163-167. https://doi. org/10.31348/2023/910.31348/2023/9 Spaide RF. Age-related choroidal atrophy. Am J Ophthalmol.2009;147:801-810. Nishida Y, Fujiwara T, Imamura Y, Lima LH, Kurosaka D, Spaide RF. Choroidal thickness and visual acuity in highly myopic eyes. Retina.2012;32:1229-1236. Hosoda YI, Uji A, Hangai M. Relationship between retinal lesions and inward choroidal bulging in Vogt-Koyanagi-Harada disease. Am J Ophthalmol.2014;157:1056-1063. Sezer T, Altınışık M, Koytak İA, Özdemir MH. Koroid ve Optik Koherens Tomogra. Turk J Ophthalmol. 2016;46:30-37. De Moraes CG, Reis AS, Cavalcante AF, Sano ME, Susanna R Jr. Choroidal expansion during the water drinking test. Graefes Arch Clin Exp Ophthalmol. 2009;247:385-389. Yannuzzi LA. Indocyanine green angiography: a perspective on use in the clinical setting. Am J Ophthalmol. 2011;151:745-751. Manjunath V, Taha M, Fujimoto JG, Duker JS. Choroidal thickness in normal eyes measured using Cirrus-HD optical coherence tomography. Am J Ophthalmol. 2010;150:325-329. Yurtoğulları Ş., Erbahçeci İE, Eyidoğan D. Migren Hastalarındaki Retina Kalınlık Değişiklikleri. Turk J Neurol. 2021;27:69-74. Spaide RF, Koizumi H, Pozzoni MC. Enhanced depth imaging spectral-domain optical coherence tomography. Am J Ophthalmol. 2008;146:496-500. Waghamare SR, Mittal S, Pathania M et al. Comparison of choroidal thickness in systemic hypertensive subjects with healthy individuals by spectral domain optical coherence tomography. Indian J Ophthalmol. 2021;69[5]:1183-1188. doi: 10.4103/ijo. IJO_1994_2010.4103/ijo Giannaccare G, Pellegrini M, Sebastiani S et al. Choroıdal Vascularıty Index Quantıfıcatıon In Geographıc Atrophy Usıng Bınarızatıon Of Enhanced-Depth Imagıng Optıcal Coherence Tomographıc Scans. Retina. 2020;40(5):960-965. Shin JW, Shin YU, Cho HY. Measurement of choroidal thickness in normal eyes using 3D OCT-1000 spectral domain optical coherence tomography. Korean J Ophthalmol. 2021;26:255-9. Li XQ, Larsen M, Munch IC. Subfoveal choroidal thickness in relation to sex and axial length in 93 Danish university students. Invest Ophthalmol. Vis Sci. 2011;52:8438-8441. Polat O.A, Sinim N, Öner AÖ. Türkiye’deki sağlıklı bireylerde spectral-domain optik koherens tomografi ile santral koroidal ve makülar kalınlık ölçümü. Ret-Vit.2012; 20:95-98. Tuncer I, Karahan E, Zengin MO, Atalay E, Polat N. Choroidal thickness in relation to sex, age, refractive error, and axial length in healthy Turkish subjects. Int Ophthalmol. 2015;35(3):403-410. doi: 10.1007/s10792-014-9962-410.1007/s10792-014-9962-4 Coşkun E, Okumuş S, Gürler B et al. Choroidal thickness in healthy Turkish subjects. Turk J Med Sci. 2014;44(1):56-61. Bhayana AA, Kumar V, Tayade A, Chandra M, Chandra P, Kumar A. Choroidal thickness in normal Indian eyes using swept-source optical coherence tomography. Indian J Ophthalmol. 2019;67(2):252-255. Wakatsuki Y, Shinojima A, Kawamura A, Yuzawa M. Correlation of Aging and Segmental Choroidal Thickness Measurement using Swept Source Optical Coherence Tomography in Healthy Eyes. PLoS One 2015;3;10(12):e0144156. Ikuno Y, Kawaguchi K, Nouchi T, Yasuno Y. Choroidal thickness in healthy Japanese subjects. Invest Ophthalmol Vis Sci. 2010;51:2173-2179. Margolis R, Spaide RF. A pilot study of enhanced depth imaging optical coherence tomography of the choroid in normal eyes. Am J Ophthalmol. 2009;147:811-815. Manjunath V, Taha M, Fujimoto JG. Choroidal thickness in normal eyes measured using Cirrus HD optical coherence tomography. Am J Ophthalmol. 2010;150:325-329. Ikuno Y, Tano Y (2009) Retinal and choroidal biometry in highly myopic eyes with spectral-domain optical coherence tomography. Invest Ophthalmol Vis Sci. 2009;50:3876-3880. Chen, X., Huang, Y., Chen, H. Distribution and Characteristics of Ocular Biometric Parameters among a Chinese Population: A Hospital-Based Study. Ophthalmol Ther. 2023;12,2117-2131. Sun Y, Wei S, Li S, Cao K, Hu J, Yang X. Distribution of ocular biometry in young Chinese eyes: the Anyang University Students Eye Study. Acta Ophthalmol. 2021; 99(6):621-627. Xie R, Qiu B, Chhablani J and Zhang X. Evaluation of Choroidal Thickness Using Optical Coherent Tomography: A Review. Front Med. 2021;8:783519. Chua, J., Tham, Y.C., Tan, B. Age-related changes of individual macular retinal layers among Asians. Sci Rep. 2019;9:20352. Ding X, Li J, Zeng J et al. Choroidal thickness in healty Chinese subjects. Invest Ophthalmol Vis Sci. 2011;20;52(13):9555-9560.